.The woman, in her 50s, was taken to hospital following the collision on the A16 Spalding bypass but sadly later died.
Three men were also taken to hospital following the two-vehicle collision, two with serious injuries.
Lincolnshire Police are appealing for anyone who may have seen the incident, which happened at around 3.24pm on Saturday.
The force is also keen for anyone who may have dashcam footage from close to the location at around the time the crash took place.
A force spokesperson said: “We received a call at 3.24pm yesterday (26th December) reporting a two-vehicle collision on the road between the McDonald’s roundabout and the industrial estate.
“It involved a black Mercedes-Benz 200 and a blue Suzuki Swift.
“The driver of the Suzuki, a woman aged in her 50s from the Boston area, was taken to hospital but later died.
“Two other occupants of the Suzuki, both men, were also taken to hospital with serious injuries not thought to be life-threatening.
“The driver of the Mercedes-Benz, a man, was taken to hospital with injuries which are not believed to be serious.
“Part of the A16 was closed following the collision and re-opened around 10.40pm.
“If you have information or dashcam footage, you can contact us in one of the following ways:
· Call 101, quoting Incident 215 of 26th December;
· Email [email protected] putting “Incident 215 of 26th December” in the Subject line;
· Contact Crimestoppers, anonymously, on 0800 555111.”