One of the country’s largest cranes demolished the bridge to expand Derbyshire County Council’s Markham Vale regeneration site.
The plans are being funded as part of a £14.2 million cash injection from the government through its Enterprise ZOne grant.
Coun Joan Dixon, cabinet member for jobs, economy and transport, said: “Markham Vale has already provided a great jobs boost to Derbyshire and we are very excited to be expanding the site further.
“The expansion is part of our long-term vision of creating 5,000 jobs for local people on both sites and we look forward to welcoming businesses by March 2015.”
More than 560 new jobs have already been created through the regeneration of the site which currently houses 28 businesses.
A further 200 jobs have already been announced with the construction of new buildings underway.
A shuttle bus will be running to provide public transport links between Poolsbrook and the Staveley and Duckmarton area, providing a connection to existing bus services while the work takes place.