The gesture was the idea of club owner Jason Clark, who has worked with the charity in his guise as CEO of Chesterfield company Proact.
Tigers donated the entire gate receipts from their game against FC United of Manchester, along with the half-time draws.
And volunteers from the charity were on hand for a bucket collection.
Rebecca Staden, senior fundraiser at The Children’s Hospital Charity, told the Guardian the money would be put to very good use.
“We want to say a special thank you to Worksop Town FC for their kind gesture and to all the fans who turned out on Saturday to support our Make it Better appeal.”
“The Children’s Hospital in Sheffield treats children from all over the region, the UK and beyond , including many children from Worksop.”
“With the money raised at the game we can help provide our young patients and their families with a world-class environment, to match the world-class care offered at our hospital. “
The cash will go towards the charity’s Make it Better appeal, which aims to raise £20m towards a £40m development at The Children’s Hospital.
It is hoped that the development will transform the building into a state-of-the-art facility with single rooms and en-suite facilities, a special play tower, gardens, and specialist medical equipment.
Visit www.make-it-better.org.uk for more information.